The Grand Princess of Tuscany was the spouse of the Grand Prince of Tuscany, heir to the Tuscan throne.
| Picture | Name | Father | Birth | Marriage | Became Princess | Ceased to be Princess | Death | Spouse |
|---|---|---|---|---|---|---|---|---|
| Joanna of Austria | Ferdinand I, Holy Roman Emperor (Habsburg) |
24 January 1547 | 18 December 1565 | 21 April 1574 became Grand Duchess |
11 April 1578 | Francesco de' Medici | ||
| Maria Maddalena of Austria | Charles II, Archduke of Austria (Habsburg) |
7 October 1589 | 19 October 1608 | 17 February 1609 became Grand Duchess |
1 November 1631 | Cosimo de' Medici | ||
| Marguerite Louise d'Orléans | Gaston, Duke of Orléans (Orléans) |
28 July 1645 | 20 June 1661 | 23 May 1670 became Grand Duchess |
17 September 1721 | Cosimo de' Medici | ||
| Violante Beatrice of Bavaria | Ferdinand Maria, Elector of Bavaria (Wittelsbach) |
23 January 1673 | 9 January 1689 | 31 October 1713 husbands death |
30 May 1731 | Ferdinando de' Medici | ||
| Anna Maria Franziska of Saxe-Lauenburg | Julius Francis, Duke of Saxe-Lauenburg (Ascania) |
13 June 1673 | 2 July 1697 | 31 October 1723 | 31 October 1723 became Grand Duchess |
15 October 1741 | Gian Gastone de' Medici | |